Haemonchus contortus is the most important bloodsucking gastrointestinal nematode in small ruminants worldwide. It is often controlled by anthelmintics. Even though other methods to control are available, the most efficient way to treat an animal with clinically manifested haemonchosis is to use one of the available broad spectrum anthelmintics: benzimidazoles (BZ), Macrocyclic lactones (ML) or imidothiazoles-tetrahydropyromidines or the narrow spectrum drugs salisylanilides or nitrophenols. Anthelmintic resistance (AR) against all groups of anthelmintics in nematode parasites has been reported from many countries, and even multiple AR has been observed. In this study I have investigated the resistance levels to all major classes of broad spectrum anthelmintics in a naturally infected flock of sheep of Red Maasai sheep (RM), Dorper sheep (D) and their offsprings on a research farm in Kenya.

Infection with Parascaris equorum is common among young horses in Sweden as well as in the rest of the world. The parasite has a direct lifecycle and reproduces in the small intestine. The eggs are passed out with faeces and become infective after approximately two weeks. The horses get infected when they ingest eggs that are dispersed in the environment. The symptoms seen in horses usually include retarded growth and loss of weight.

P-glykoprotein (P-gp) är en effluxtransportör som ursprungligen uppmärksammades för sin kapacitet att skydda tumörceller från flertalet läkemedel. Det har sedan visat sig att P-gp även finns i andra vävnader, som tarm, njurtubuli, lever samt i hjärnans kapillära endotelceller. P-gp är en viktig komponent i blod-hjärnbarriären där den hindrar passage av icke-essentiella substanser till hjärnan. Genen som kodar för P-gp kallas MDR1. Mutationen som flertalet hundraser har är en deletion i det fjärde kodande exonet i denna gen.

The purpose of this study was to evaluate enantioselective analytical methods by separation of the enantiomers of four drugs (citalopram, zopiclone, tramadol and methylphenidate) and their metabolites. The analyses were performed with HPLC-UV with columns whose stationary phases were based on macrocyclic glycopeptides (Chirobiotic V, V2 and T) and polysaccharides (Lux Cellulose-1, Cellulose-2 and Amylose-2).The Chirobiotic V column showed high selectivity for citalopram and its metabolites. High resolution was obtained using a mobile phase consisting of methanol, acetic acid and ammonia. High selectivity for the enantiomers of zopiclone and its metabolites were obtained on the Cellulose-2 column using a mobile phase consisting of acetonitrile and ammonium acetate buffer.The enantiomers of tramadol were separated with the Amylose-2 column. However, changes in the pressure arose, probably caused by the additive NH4HCO3.

Horses are herbivores that spend almost all day grazing. While grazing they are infected by different endoparasites through ingestion of infective eggs or larvae on pasture. The most significant equine endoparasites in Sweden are the equine roundworm Parascaris equorum, small (Cyathostominae) and large strongyles (Strongylus spp), the tapeworm Anoplocephala perfoliata and the pinworm Oxyuris equi. For many years have horses been dewormed on regularly basis, which has resulted in the development of resistance to many anthelmintic classes. Because of the problems with resistance it is since 2007 necessary to have a prescription on anthelmintics and faecal sampling is also recommended.